Conference Schedule

Day1: October 11, 2018

Keynote Forum

Biography

Guy Wouters, Bio-Engineer (KU Leuven) and Veterinary Scientist (RUGhent),has been working since long in biotech for health care at different universities and companies. As an Expert in cellular therapies, he organized a state of the art production unit, conforming to GMP requirements for quality and hygiene. He is the Director of Fat-Stem Laboratories, one of the few companies that conducts research in the therapeutic use of adipose-derived mesenchymal stem cells (ASC) and was the first biotech company in the European Union to offer commercial ASC based therapy for horses with ligament and tendon injuries, as well as for joint disease in horses. He has published more than 50 papers in reputed journals and has been serving as a Board Member of different biotech companies.

 

Abstract

Conventional therapies for SCCED as corneal erosion or corneal ulcers are the use of antibiotics, ointments, simple debridement or sewing of the eyelids together to protect the eye surface. The conventional treatments for SCCED unfortunately are not always very successful. Innovative methods of treatment are developed and in this study the potential of regenerative corneal therapy is investigated. Evidence has accumulated that growth factors such as Epidermal Growth Factor (EGF), Fibroblast Growth Factor (FGF), Transforming Growth Factor (TGF) and Insulin-Like Growth Factor (IGF) play a key role in corneal healing. These growth factors promote the proliferation and induce the migration of corneal cells. Inflammatory cytokines such as interleukin IL-1, IL-6 and Tumor Necrosis Factor-alpha (TNF-α) are also involved in corneal regeneration and improve corneal transparency. This study was conducted in collaboration with Ghent University to prove the effectiveness of Eye-Regen® (Fat-Stem Laboratories, Belgium) by treating five dogs of different breeds (two Golden Retrievers, one French Bulldog, one Jack Russell and one Canis vulgaris with SCCED. The dogs initially went through conservative and/or chirurgical treatments for a period of 3–4 months and showed poor clinical improvement and finally relapse. Eye-Regen® is a newly formulated product with growth factors originated from stem cells and has the ability to rehydrate dry eyes and promote rapid healing of the corneal structure. After previous conservative treatments, Eye-Regen® were applied (4–6 drops/day during 10 days after which application was gradually phased out). All dogs showed removal of clinical symptoms or complete healing after minimum two months, compared to little or no improvement after surgical treatment combined with classical drugs. Adverse effects were not reported. This case study reveals that this innovative regenerative treatment can be an indication for use in most SCCEDs as conjunctivitis and Kerato Conjunctivitis Sicca (KCS), trauma (eye edge), keratitis, corneadegeneration, ulcerations and other necrotic eye injuries.

                       

Figure 1: Before and after 1 month of treatment with Eye-Regen®.

                         

Figure 2: Before and after 1 month of treatment with Eye-Regen®

                          
Figure 3: Eye-Regen® are provided in sterile vials of 3 ml (with droplet
counter).

 

Biography

P V Tresamol has her expertise in the field of Veterinary Epidemiology and Preventive Medicine. During the period of 24 years of service as a Faculty in the Kerala Veterinary and Animal Sciences University, she was associated with Teaching, Research and Extension activities in her field. She has handled 20 research projects as Principal/Co-Principal Investigator and has guided 40 PG Scholars and two Doctorate Scholars as Major/Minor Advisor. She was instrumental in organizing seminars, training programs, health camps and disease outbreak investigations in the university. She has attended several national and international training programs /seminars and published around 120 research articles in various national and international journals. Currently she is in the Associate Dean of College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala.

 

Abstract

Statement of the Problem: Dermatophilosis or cutaneous streptothricosis, caused by D. congolensis is an acute or chronicdisease which affects mostly ruminants and horses. The diseaseis of great economic significance as it results in considerable loss due to reduced production, downgrading of hide, increased rate of culling and death. Rapid and accurate diagnosis of dermatophilosis is required for a prompt and proper management of the condition. Cultural isolation of the organisms from skin scabs and then identification by PCR takes minimum of 48 to 72 hours. Hence, the aim of this study was to standardize the PCR technique using DNA extracted directly from the skin scabs collected from lesions. Methodology & Theoretical Orientation: Skin scabs were collected from animals suspected for dermatophilus dermatitis such as matting of hairs, thick scabs with cracks and fissures and were confirmed using conventional bacteriological examination. Samples were collected from cattle, buffaloes, goat, horse and camel with suggestive lesions. DNA extraction was carried out from the cultures as well as directly from the scabs and PCR was carried out using the species specific primers targeting 16S rRNA of D. congolensis. DNA sequencing was done and homology searches were performed with the NCBI database. Findings: Polymerase chain reaction yielded specific 500 bp amplicons from all the skin scab samples from the positive cases of cattle, buffaloes, goats, horses and camel as well as from cultures. Results could be obtained within five to six hours when scabs are used directly, whereas it took 48–72 hours for cultural isolation and subsequent amplification. Moreover direct amplification of 16S rRNA gene from scab samples was useful in the diagnosis of infection, especially in cases of recovering animals, chronically infected animals or wet scab where the organisms generally exist as scattered cocci rather than the characteristic tram-track pattern. This will make the diagnosis easy and also gives a rapid confirmatory diagnosis

 

Tracks

  • One Health | Veterinary Dermatology | Veterinary Pharmaceuticals | Animal Health | Veterinary Medicine | Veterinary science | Animal Models and Testing
Location: Tamdhu
Speaker

P.V. Tresamol

Kerala Veterinary and Animal Sciences University, India

Chair

Speaker

Zaneta Dziegelewska

Warsaw University of Life Sciences, Poland

Co Chair

Biography

David J Wilson was born and grew up in Wisconsin. Graduated from veterinary school at Ohio State University in 1982. Practiced in mixed practice, mainly dairy, until 1987. Three-year residency and post-DVM MS specializing in mastitis and udder health completed in 1990 from Michigan State University. Worked in mastitis and udder health at Cornell University for 15 years, also earning a PhD in Epidemiology and Immunology in the Employee Degree Program. Came to Utah State University in 2006 as dairy extension veterinarian and epidemiologist for the Utah Veterinary Diagnostic Laboratory. Worked mainly on mastitis, milk quality, and stray voltage on dairy farms, high throughout disease testing using milk samples, bovine immunology and Johne’s disease. Other work has included Bovine Viral Diarrhea and epidemiology of disease in many species tested through the Utah Veterinary Diagnostic Laboratory.

 

Abstract

The objective of this study was to compare conventional microbial culture, MALDI–TOF (Matrix Assisted Laser Desorption/Ionization– Time of Flight), and 16S rRNA partial genomic sequencing methods for microbial identification in quarter milk samples from dairy cattle. The same microbial colonies were tested using each method. There is no agreed upon “gold standard” for “true positive” microbial identification. Therefore, this was a study of test agreement, not sensitivity or specificity; the latter calculations require “true” disease status. All 181 bacterial isolates were tested by culture and MALDI–TOF, while 179 were tested by 16S rRNA because two isolates were lost during storage before the latter test was performed. For Staphylococcus aureus and Escherichia coli, agreement was to the species level in accordance with conventional culture. For all other microbes, agreement was defined as to the genus level or to within the group defined as streptococcal-like organisms,in keeping with culture and accepted industry practices. All samples were mycoplasma-negative. Overall agreement in identification of microbes between all three diagnostic methods was 94% (169/179). Agreement between MALDI–TOF and 16S rRNA was 98% (176/179); culture agreement with each of the other two methods was 95%. Specific microbes were identified with agreement among all three methods ranging from 97% to 100%, all classified “very good” by the Kappa test. Many members of the dairy industry are used to either bacteriological culture or MALDI–TOF for routine mastitis pathogen diagnosis, and there is interest in the agreement between the methods. These results suggest that either method is of practical value. At present 16S rRNA testing is primarily a research tool, but it showed high agreement with the other methods. For purposes of milk quality and udder health monitoring or study any of the three methods are valuable tools for the dairy industry

 

Biography

C Latha, Dean of College of Veterinary and Animal Sciences has 26 years of research experience and has completed 25 research projects funded by many funding agencies like ICAR. She has published 70 research articles in reputed national and international scientific journals, three books and has presented 150 papers in several national and international seminars and symposiums. She is holds key positions as the University Head of the Department of Veterinary Public Health, Chairman of Project Coordination Committee, Board of Studies and Faculty of Research Council of the university.

Abstract

Statement of the Problem: Toxoplasmosis is emerging as a major foodborne disease of man and animals across the world. A ‘one-health’ approach is essential to understand, prevent, and control toxoplasmosis, while the gaps in the epidemiological information targeting human, animal and environmental counterpart have been found in earlier studies. As a one health collaborative approach, the purpose of this study is to determine the prevalence of toxoplasmosis in felines, caprines, humans and environmental samples in Thrissur, Kerala. Methodology & Theoretical Orientation: A total of 704 samples comprising of feline faeces, caprine serum, caprine milk, human serum, soil and water were collected from Thrissur, Kerala. The samples were subjected to detection of the Toxoplasma using different methods such as oocyst detection and molecular characterization using B1 gene in feline samples, soil and water samples and seroprevalence using IgG ELISA in caprine and human samples. The different risk factors associated with T. gondii infection in caprines and humans were also analyzed using standardized questionnaire. Findings: The T. gondii oocyst was detected in 4.47% of 313 feline faecal samples. On molecular detection by PCR, the B1 gene of Toxoplasma sp. was detected in six out of 61 soil samples. None of the 61 water samples showed presence of the parasite. Overall seroprevalence of 41.30% was observed in 184 caprine sera and 52.46% was recorded in the 161 human serum samples. A higher seroprevalence was observed in caprines of >4 years of age and with grazing habit and in ewes with history of bad obstetrics. Practice of rearing of more than three cats and those humans involved in gardening and agricultural activities had higher prevalence of T. gondii antibodies. Seroprevalence of the parasite in older individuals and in women with history of gynaecological disease conditions and on non-vegetarian diet was higher. Conclusion & Significance: The study indicates the wide spread presence of the parasite in the area. This necessitates the implementation of effective surveillance and monitoring system for the detection of food borne parasitic disease globally and one health approach is inextricably linked to the control of the disease.

Biography

Talia M Tremori has her expertise in Veterinary Medicine, Animal Pathology applied to Forensic Sciences. Bechelor’s degree in Veterinary Medicine, master’s degree in Animal Pathology (Sao Paulo State University, Campus of Botucatu, Brazil) doctor’s degree in Veterinary Preventive Medicine (Sao Paulo State University, Campus of Botucatu, Brazil) and Health and Development on the tropics (University of Salamanca, Spain), researching criminal identification of animals coming from trafficking and its impact on public health. She integrates the “Pro Forenses” Project (CAPES–Brazil), WAWFE (Worldwide Association of Women Forensics Experts) and “Association Ibero american of Medicine and Forensic Veterinary Sciencess”. She has participated in many events in Brazil and abroad in Forensic Veterinary Medicine, Animal Science and Forensic Sciences.

Abstract

Trypanosoma cruzi, an important protozoan parasite for humans and animals, causes Chagas disease, a Neglected Tropical Disease (NTD) that could affect roughly 6–7 million people in the world, mainly in underdeveloped countries. The vector is a blood-sucking insect and so many mammals could be reservoirs. Animal trafficking, smuggling and illegal trade is the fourth most common illegal activity in the world. An important point concerning illegal animal trade and the increasing globalization is that it represents a possible vehicle for illness spreading, including zoonosis, creating a health public issue. Hence, the diagnosis in endemic regions and limited resources is very important. An alternative is a molecular technique named Loop-Mediated Isothermal Amplification (LAMP), and this assay is a one-step amplification reaction that amplifies a target DNA with high specificity, efficiency and rapidity under isothermal conditions. This study verified the prevalence of the zoonotic agent T. cruzi in 50 samples from animal trafficking of Brazilian mammals, using muscle, blood and skin samples. The molecular diagnosis of T. cruzi was done using the LAMP assay and had been 50% of positivity according to figure 1. This analysis could be important to identify reservoirs of T. cruzi and the risk about animal trafficking to human health. Also the use of LAMP assay in fast and trial diagnosis to major infections zoonotic diseases.

                           
Figure 1: Frequency distribution of LAMP positivity on animal trafficking
samples (N = 50).

Biography

Qamar-un-Nisa, is serving as Assistant Professor in Department of Pathology. Faculty of Veterinary Science, University of Veterinary & Animal Sciences (UVAS) Lahore, Pakistan. She is graduate in veterinary sciences from University of Agriculture, and M. Phil in Pathology from UVAS. Now she has done PhD in the discipline of Pathology UVAS, Lahore funded by Higher Education Commission, Government of Pakistan. She has over 11 years teaching and research experience. She is involved in teaching, research, clinical & laboratory. She is the member of various scientific boards. She has attended many conferences and seminars nationally and internationally. She has also attended 5th Turkish Vet. Pathology Congress, Bursa-Turkey and orally presented her research paper on ‘’Histopathological and hematological analysis of broiler chicken experimentally infected with pathogenic E.coli. She has more than 15 national and international scientific publications.

Abstract

Respiratory diseases are responsible for major economic losses in poultry farms. Newcastle Disease Virus (NDV) infections cause huge economic losses in poultry industry especially in the presence of other co-infecting pathogens. The purpose of this study was to assess the less understood effect of Mycoplasma gallisepticum (MG) on the replication and immune responses of NDV in broiler chicken. Three-weekold commercial broiler chickens were inoculated with NDV, MG or both etiological agents. The experimental groups were identified as follows: Negative control (Group C), Mycoplasma challenged (Group M), NDV challenged (Group V) and virus and Mycoplasma challenged (Group V+M). Blood samples and swabs were collected on daily basis for two weeks. All infected birds showed positive results for NDV shedding, however, the pattern of virus shedding was different with birds of the group V+M showing more pronounced virus shedding than the birds in the group V. In addition, birds of V+M group showed significant reduction in anti-AI antibody responses and interferon gene expression than the birds in the V group. The present study revealed that MG could facilitate replication of NDV by bringing alterations in immune responses.

Biography

Talia M Tremori has her expertise in Veterinary Medicine, Animal Pathology applied to Forensic Sciences. Bechelor’s degree in Veterinary Medicine, master’s degree in Animal Pathology (Sao Paulo State University, Campus of Botucatu, Brazil) doctor’s degree in Veterinary Preventive Medicine (Sao Paulo State University, Campus of Botucatu, Brazil) and Health and Development on the tropics (University of Salamanca, Spain), researching criminal identification of animals coming from trafficking and its impact on public health. She integrates the “Pro Forenses” Project (CAPES–Brazil), WAWFE (Worldwide Association of Women Forensics Experts) and “Association Ibero american of Medicine and Forensic Veterinary Sciencess”. She has participated in many events in Brazil and abroad in Forensic Veterinary Medicine, Animal Science and Forensic Sciences.

Abstract

Animal humanization emerged in the last few decades, it is important that the veterinarian has forensic medicine knowledge as to identify possible injuries and non-accidental trauma that the animal can be submitted to which is not always easily identified. Furthermore, the animal humanization and judicial control are factors that turned the need of veterinarian in courts and as assistants of justice each time more usual. Several occurrences relate to crimes against animals, such as trafficking, abandonment, maltreatment, physical aggression, bestiality and zoophilia, animal hoarders and yet situations where the animal is the first victim to be reached before humans. Hence the link theory was created, aiming to establish a link between anima violence and other vulnerable groups, like women in the domestic environment, elderly and children. According to World Health Organization (WHO) the veterinarian constitutes as adequate of health for being capable of preventing the population of collective diseases. Moreover, cases that occur in the family field regarding domestic violence can be highlighted. This kind of situation occurs for instance, when a physical aggression happens and the perpetrator ritualizes and frightens the victim through threats and cruel acts towards the pets. In these terms, violence against the animal is also a significant social affair. Animal crimes deserve attention not only upon the law, but also as a wholesome society, through professionals prepared to act within any segment, including police officers. Overall, features the importance of interdisciplinary and the immersion of veterinary medicine within the forensics context, both in the training of the clinical veterinary as to guide and identify cases and to the pathologist to diagnose injuries and elaborate legal medical records that can contribute to clarify crimes against animals.

Figure 1: Relationship between animal abuse, child maltreatment, elder abuse and domestic violence, used to explain “link coalition”.

 

 


 

 

Biography

Nesreen M Safwat, Bachelor in Veterinary Medicine, Faculty of Veterinary Medicine. Beni-Suef Univ., (2006) and general grade: Very good with degree of honor ranking: second over my class. Master degree of Pathology, College of Veterinary Medicine, Beni-Suef University (2011) Thesis title: “Pathological studies on skin lesions in ruminants”, PhD of pathology (2015) thesis entitled: “Pathological studies on ovarian alterations in relation to genital tract in bovine”. Her experience as senior analyst in Salah Deeb´s histopathology lab Beni-Suef University. Her experience in studying of some methods aiding in histopathology techniques.

Abstract

The mounting medium is a solution used to adhere the coverslip to the slide to preserve and support stained tissue sections. The most popular mounting media used in laboratories for histopathology is a mixture of Distyrene (apolystyrene), a Plasticiser (tricresyl phosphate), and Xylene called DPX mounting medium that preserves stains and dries quickly. In this study a novel mixture was used replacing DPX; a mixture of extra pure benzene and Expanded Polystyrene Foam (EPF) which mixed together forming adhesive material used to adhere the coverslip with the clean, stained tissue slides, then examined under light microscopy by using different objective lenses magnification in comparison with DPX which cover the same stained tissue sections. Stored for one year to monitor the fading effect of the new mountant on the stained tissue sections. In conclusion Getting the same results of DPX, specially is a simple mixture can be made by any technician in any laboratory for histopathology as well as the time elapsed for drying of the new mounting medium is much more rapid in comparison with DPX and also the spread of EPF in many daily use product make the mixture also available and recycled in a good way as EPF used in floatation devices, egg cartons, sandwich and hamburger boxes, coffee cups and plates. Finally when I made comparison between this new mixture and DPX using the same tissues, the great results was the same, also when the coloures has been measured by image analyzer software to the record the difference.

  
the new mounting mixture in comparison with DPX.Plate: Showing microphotographs of rat liver by different magnifications of

Biography

Nejash Abdela did an excellent academic achievement and received a degree of Doctor of Veterinary Medicine (DVM) with great distinction from Jimma University in 2017. He is also a well-versed young energetic researcher with admirable scientific paper writing and publication skills. Inspiringly he has published more than 40 scientific papers on reputable peer reviewed international journals. Moreover, he is the reviewer and editorial board member of many international journals.

Abstract

Dairy producer increase milk production by over feeding grain diets that are high in starch and low in fiber to increase intake of energy and met dietary requirements of the high yielding dairy cows. However, these diets increase the risk of Sub Acute Ruminal Acidosis (SARA). Thus, maximizing milk production without incurring Sub-acute ruminal acidosis is a challenging most dairy producers. The main aims of this paper were to review available article on general aspects of Subacute ruminal acidosis and its consequence in dairy cattle by focusing on past and recent article and helping to update the current knowledge for early recognition and limit the associated negative impact in dairy industry. Sub-acute ruminal acidosis is a well-recognized and economically important digestive disorder found particularly in well-managed dairy cattle. It is a consequence of feeding high grain diets to dairy cows and characterized by daily episodes of low ruminal which generally occurs when ruminal pH stays in the range of 5.2 and 6 for a prolonged period resulting in depresses fiber digestion and possibly milk production. There is no typical clinical sign of illness in SARA affected cows. However, SARA is said to be associated with inflammations of different organs and tissues in dairy cows. Rumenocentesis remains the most reliable means of diagnosing SARA. The cow at risk to develop SARA includes cows in the early lactation, Primiparous cows and Cows grazing or fed with rapidly fermentable low fiber grass. SARA has long-term health and economic consequences, which include feed intake depression, fluctuations in feed intake, reduce  diet digestibility, reduced milk yield, reduced milk fat percent, gastrointestinal damage, liver abscesses and lameness. Apart from compromises to dairy cow health and economics, SARA is of concern for animal welfare reasons, since lameness and laminitis impact significantly on cow comfort and general wellbeing  Thus, it represents a significant concern for dairy industry and the cattle should be regularly monitored to facilitate early recognition of the condition and limit the associated economic losses.